Publications

* = equal contribution
✉ = corresponding author
bold = Pilhofer Lab members

Full List of Publications

Preprints

Casu B, Sallmen JW, Haas PE, Afanasyev P, Xu J, ✉Schlimpert S, ✉Pilhofer M
Firing of Streptomyces coelicolor contractile injection system requires the membrane protein CisA
external page Biorxiv

Bianco E, Bonassera M, Uliana F, Tilma J, Winkler M, Zencir S, Albert B, Oborská-Oplová M, Dechant R, Hugener J, Panse VG, Pilhofer M, Kimmig P, ✉Peter M
Stm1 regulates Ifh1 activity revealing crosstalk between ribosome biogenesis and ribosome dormancy
external page Biorxiv

2024

Zachs T, Malit JJL, Xu J, Schürch A, Sivabalasarma S, Nußbaum P, Albers SV, ✉Pilhofer M
Archaeal type six secretion system mediates contact-dependent antagonism
external page Science Advances

Lien YW, Amendola D, Lee KS, Bartlau N, Xu J, Furusawa G, Polz MF, Stocker R, ✉Weiss GL, ✉Pilhofer M
Mechanism of bacterial predation via ixotrophy
external page Science | Read the external page Perspective

Kohtz AJ, *Petrosian N, *Krukenberg V, *Jay ZJ, Pilhofer M, ✉Hatzenpichler R
Cultivation and visualization of a methanogen of the phylum Thermoproteota
external page Nature

*Hugener J, *Xu J, Wettstein R, Ioannidi L, Velikov D, Wollweber F, Henggeler A, ✉Matos J, ✉Pilhofer M
FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis
external page Cell

*Wettstein R, *Hugener J, *Gillet L, *Hernández-Armenta Y, Henggeler A, Xu J, van Gerwen J, Wollweber F, Arter M, Aebersold R, ✉Beltrao P, ✉Pilhofer M, ✉Matos J
Waves of regulated protein expression and phosphorylation functionally rewire the proteome to drive gametogenesis
external page Developmental Cell

✉Gaisin VA, van Wolferen M, Albers SV, ✉Pilhofer M
Distinct life cycle stages of an ectosymbiotic DPANN archaeon
external page ISME Journal

Feldmüller M, *Ericson CF, *Afanasyev P, *Lien YW, Weiss GL, Wollweber F, Schoof M, Hurst M, ✉Pilhofer M
Stepwise assembly and release of Tc toxins from Yersinia entomophaga
external page Nature Microbiology

2023

✉Pilhofer M
Announcement: Journal of Structural Biology – Paper of the Year 2022
external page Journal of Structural Biology

Costafrolaz J, Panis G, Casu B, Ardissone S, Degeorges L, Pilhofer M, ✉Viollier PH
Adaptive β-lactam resistance from an inducible efflux pump that is post-translationally regulated by the DjlA co-chaperone
external page PLoS Biology

Hüsler D, Stauffer P, Steiner B, Böck D, Steiner T, Kroniger T, Striednig B, Swart AL, Letourneur F, Maaß S, Becher D, Eisenreich W, Pilhofer M, ✉Hilbi H
The large GTPase Sey1/atlastin mediates lipid droplet- and FadL-dependent intracellular fatty acid metabolism of Legionella pneumophila
external page Elife

Ericson CF, ✉Pilhofer M
Mix-and-match tools for protein injection into cells
external page Nature

Casu B, Sallmen JW, ✉Schlimpert S, ✉Pilhofer M
Cytoplasmic contractile injection systems mediate cell death in Streptomyces
external page Nature Microbiology | Read the external page Perspective

Wohlfarth JC, Feldmüller M, Schneller A, Kilcher S, Burkolter M, Pilhofer M, Schuppler M, ✉Loessner MJ
Gram-positive bacteria evade phage predation through endolysin-mediated L-form conversion
external page Nature Microbiology

2022

*Rodrigues-Oliveira T, *Wollweber F, *Ponce-Toledo RI, Xu J, Rittmann SKMR, Klingl A, ✉Pilhofer M, ✉Schleper C
Actin cytoskeleton and complex cell architecture in an Asgard archaeon
external page Nature | Read the external page News & Views and press release

*Kieninger AK, *Tokarz P, Pilhofer M, ✉Weiss GL, ✉Maldener I
SepN is a septal junction component required for gated cell–cell communication in the filamentous cyanobacterium Nostoc
external page Nature Communications

*Weiss GL, *Eisenstein F, Kieninger AK, Xu J, Minas HA, Gerber M, Feldmüller M, Maldener I, Forchhammer K, ✉Pilhofer M
Structure of a thylakoid-anchored contractile injection system in multicellular cyanobacteria
external page Nature Microbiology | Read perspectives in external page ScienceNews and external page Nature Microbiology

*Xu J, *Ericson CF, Feldmüller M, Rutaganira FUN, Eisenstein F, Lien YW, King N, ✉Pilhofer M
Identification and structure of an extracellular contractile injection system from the marine bacterium Algoriphagus machipongonensis
external page Nature Microbiology | Read perspectives in external page ScienceNews and external page Nature Microbiology

2021

✉Yakimov MM, Merkel AY, Gaisin VA, Pilhofer M, Messina E, Hallsworth JE, Klyukina AA, Tikhonova EN, Gorlenko VM
Cultivation of a vampire: 'Candidatus Absconditicoccus preadator'
external page Environmental Microbiology

*Böck D, *Hüsler D, Steiner B, Medeiros JM, Welin A, Radomska KA, Hardt WD,
✉Pilhofer M, ✉Hilbi H
The polar Legionella Icm/Dot T4SS establishes distinct contact sites with the pathogen vacuole membrane
external page mBio

✉Lobato-Márquez D, Xu J, Güler GO, Ojiakor A, Pilhofer M, ✉Mostowy S
Mechanistic insight into bacterial entrapment by septin cage reconstitution
external page Nature Communications

2020

✉Springstein BL, Nürnberg DL, Weiss GL, Pilhofer M, Stucken K
Structural Determinants and Their Role in Cyanobacterial Morphogenesis
external page Life

*Stanisich JJ, *Zyla DS, Afanasyev P, Xu J, Kipp A, Olinger E, Devuyst O, Pilhofer M, Boehringer D, ✉Glockshuber R
The cryo-EM structure of the human uromodulin filament core reveals a unique assembly mechanism
external page Elife

*Weiss GL, *Stanisich JJ, *Sauer MM, Lin CW, Eras J, Zyla DS, Trück J, Devuyst O, Aebi M, 
✉Pilhofer M, Glockshuber R
Architecture and function of human uromodulin filaments in urinary tract infections
external page Science | Also read a external page Perspective and a external page Digest

*✉Gaisin VA, *Kooger R, Grouzdev DS, Gorlenko VM, Pilhofer M
Cryo-electron tomography reveals the complex ultrastructural organisation of multicellular filamentous Chloroflexota (Chloroflexi) bacteria
external page Frontiers in Microbiology

*Fattinger S, *Böck D, Di Martino ML, Deuring S, Ventayol PS, Ek V, Furter M, Kreibich S, Bosia F, Müller-Hauser AA, Nguyen BD, Rohde M, ✉Pilhofer M, ✉Hardt WD, ✉Sellin ME
Salmonella Typhimurium discreet-invasion of the murine gut absorptive epithelium
external page PLoS Pathogens

Zachs T, Schertel A, Medeiros JM, Weiss GL, Hugener J, Matos J, ✉Pilhofer M
Fully automated, sequential focused ion beam milling for cryo-electron tomography
external page Elife

Hausmann A, Böck D, Geiser P, Berthold D, Fattinger SA, Furter M, Bouman J, Barthel-Scherrer M, Lang CM, Bakkeren E, Kolinko I, Diard M, Bumann D, Slack E, Regoes RR, ✉Pilhofer M, ✉Sellin ME, ✉Hardt WD
Intestinal epithelial NAIP/NLRC4 restricts systemic dissemination of the adapted pathogen Salmonella Typhimurium due to site-specific bacterial PAMP expression
external page Mucosal Immunology

2019

*Wu CF, *Lien YW, Bondage D, Lin JS, Pilhofer M, Shih YL, Chang J, ✉Lai EM
Effector loading onto the VgrG carrier activates the assembly of the type VI secretion system
external page EMBO Reports

*Ericson CF, *Eisenstein F, Medeiros JM, Malter KE, Calvalcanti G, Zeller RW, Newman DK, ✉Pilhofer M, ✉Shikuma NJ
A contractile injection system stimulates tubeworm metamorphosis by translocating a proteinaceous effector
external page Elife | Read Alain Filloux' external page digest

Eisenstein F, Danev R, ✉Pilhofer M
Improved applicability and robustness of fast cryo-electron tomography data acquisition
external page Journal of Structural Biology

*Weiss GL, *Kieninger AK, ✉Maldener I, Forchhammer K, ✉Pilhofer M
Structure and function of a bacterial gap junction analog
external page Cell

*Rocchi I, *Ericson CF, *Malter KE, Zargar S, Eisenstein F, Pilhofer M, ✉Beyhan S, ✉Shikuma NJ
A bacterial phage tail-like structure kills eukaryotic cells by injecting a nuclease effector
external page Cell Reports

Szwedziak P, ✉Pilhofer M
Bidirectional contraction of a type six secretion system
external page Nature Communications

*Rapisarda C, *Cherrak Y, *Kooger R, Schmidt V, Pellarin R, Logger L, Cascales E, ✉Pilhofer M, ✉Durand E, ✉Fronzes R
In situ and high-resolution cryo-EM structure of the type VI secretion membrane complex
external page EMBO Journal

2018

*Kooger R, *Szwedziak P, Böck D, ✉Pilhofer M
CryoEM of bacterial secretion systems
external page Current Opinion in Structural Biology

*Cai S, *Böck D, ✉Pilhofer M, ✉Gan L
The in situ structures of mono-, di-, and tri-nucleosomes in human heterochromatin
external page Molecular Biology of the Cell

*Medeiros JM, *Böck D, Weiss GL, Kooger R, Wepf R, ✉Pilhofer M
Robust workflow and instrumentation for cryo-focused ion beam milling of samples for electron cryotomography
external page Ultramicroscopy

2017

Medeiros JM, Böck D, ✉Pilhofer M
Imaging bacteria inside their host by cryo-focused ion beam milling and electron cryotomography
external page Current Opinion in Microbiology

✉Toyofuku M, Cárcamo-Oyarce G, Yamamoto T, Eisenstein F, Hsiao C, Kurosawa M, Gademann K, ✉Pilhofer M, Nomura N, ✉Eberl L
Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis
external page Nature Communications

*Böck D, *Medeiros JM, Tsao HF, Penz T, Weiss GL, Aistleitner K, ✉Horn M, ✉Pilhofer M
In situ architecture, function, and evolution of a contractile injection system
external page Science

Weiss GL, Medeiros JM, ✉Pilhofer M
In situ imaging of bacterial secretion systems by electron cryo-tomography
external page Methods in Molecular Biology

Dobro M, Oikonomou C, Piper A, Cohen J, Guo K, Jensen T, Tadayon J, Donermeyer J, Park Y, Solis B, Kjaer A, Jewett A, McDowall A, Chen S, Chang Y, Shi J, Subramanian P, Iancu C, Li Z, Briegel A, Tocheva E, Pilhofer M, ✉Jensen G
Uncharacterized bacterial structures revealed by electron cryotomography
external page Journal of Bacteriology

2016

*Shikuma NJ, *Antoshechkin I, Medeiros JM, Pilhofer M, ✉Newman DK
Stepwise Metamorphosis of the Tubeworm Hydroides elegans is Mediated by a Bacterial Inducer and MAPK Signaling
external page PNAS

✉Guillaume-Gentil O, Grindberg RV, Kooger R, Dorwling-Carter L, Martinez V, Ossola D, Pilhofer M, Zambelli T, ✉Vorholt JA
Tunable Single-Cell Extraction for Molecular Analyses
external page Cell

---Caltech---

2014

*Shikuma NJ,*Pilhofer M, Weiss GL, ✉Hadfield MG, ✉Jensen GJ, ✉Newman DK
Marine tubeworm metamorphosis induced by arrays of bacterial phage tail-like structures
external page Science | Featured by external page The Scientist, external page Small Things Considered, external page Scientific American

*Pilhofer M, *Aistleitner K, Ladinsky MS, König L, ✉Horn M, ✉Jensen GJ
Architecture and host interface of environmental chlamydiae revealed by electron cryotomography
external page Environmental Microbiology

2013

*Pilhofer M, *Aistleitner K, Biboy J, Gray J, Kuru E, Hall E, Brun YV, VanNieuwenhze MS, Vollmer W, ✉Horn M, ✉Jensen GJ
Discovery of chlamydial peptidoglycan reveals bacteria with murein sacculi but without FtsZ
external page Nature Communications| Featured by Nature Reviews Microbiology

*Briegel A, *Pilhofer M, Mastronarde DN, ✉Jensen GJ
The challenge of determining handedness in electron tomography and the use of DNA origami helices as molecular standards
external page Journal of Structural Biology

✉Pilhofer M, and Jensen GJ
The bacterial cytoskeleton: more than twisted filaments
external page Current Opinion in Cell Biology

2012

*Basler M, *Pilhofer M, Henderson GP, ✉Jensen GJ, ✉Mekalanos JJ
Type VI secretion requires a dynamic contractile phage tail-like structure
external page Nature | Featured by Nature Reviews Microbiology, F1000

2011

✉Pilhofer M, Ladinsky MS, McDowall AW, Petroni G, ✉Jensen GJ
Microtubules in Bacteria: ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton
external page PLoS Biology| Featured by Nature’s Research Highlights, F1000, ASM Microbe

Swulius MT, Chen S, Ding HJ, Li Z, Briegel A, Pilhofer M, Tocheva EI, Lybarger SR, Johnson TL, Sandkvist M, ✉Jensen GJ
Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria
external page Biochemical and Biophysical Research Communications

2010

Pilhofer M, Ladinsky MS, McDowall AW, ✉Jensen GJ
Bacterial TEM: New insights from cryo-microscopy
external page Methods in Cell Biology

Chen S, McDowall A, Dobro MJ, Tocheva EI, Briegel A, Ladinsky M, Shi J, Beeby M,
Pilhofer M, Ding HJ, Li Z, Gan L, Morris DM, ✉Jensen GJ
Electron Cryotomography of Bacterial Cells
external page Journal of Visualized Experiments

---Technical University Munich---

2009

✉Pilhofer M
Bakterielle Tubuline und Zellteilung in Prosthecobacter
Invited review in external page BIOSpektrum - Karriere, Köpfe und Konzepte

Grünwald S, Pilhofer M, ✉Höll W
Microbial associations in gut systems of wood- and bark-inhabiting longhorned beetles.
external page Systematic and Applied Microbiology

Pavlekovic M, Schmid MC, Schmider N, Spring S, Pilhofer M, Gaul T, Fiandaca M, Löffler FE, Jetten M, Schleifer KH, ✉Lee NM
Optimization of three FISH procedures for in situ detection of anaerobic ammonium oxidizing bacteria in biological wastewater treatment
external page Journal of Microbiological Methods

Weber SD, Hofmann A, Pilhofer M, Wanner G, Agerer R, Ludwig W, Schleifer KH, ✉Fried J
The diversity of fungi in aerobic sewage granules assessed by 18S rDNA and ITS sequence analyses
external page FEMS Microbiology Ecology

✉Pilhofer M, Pavlekovic M, Lee NM, Ludwig W, Schleifer KH
Fluorescence in situ hybridization for intracellular localization of nifH mRNA
external page Systematic and Applied Microbiology

2008

✉Pilhofer M, Rappl K, Eckl C, Bauer AP, Ludwig W, Schleifer KH, Petroni G.
Characterization and evolution of cell division and cell wall synthesis genes in the bacterial phyla Verrucomicrobia, Lentisphaerae, Chlamydiae and Planctomycetes and phylogenetic comparison with rRNA genes
external page Journal of Bacteriology

2007

Pilhofer M, Bauer AP, Schrallhammer M, Richter L, Ludwig W, Schleifer KH, ✉Petroni G
Characterization of bacterial operons consisting of two tubulins and a kinesin-like gene by the novel Two-Step Gene Walking method
external page Nucleic Acids Research

Pilhofer M, Rosati G, Ludwig W, Schleifer KH, ✉Petroni G
Coexistence of tubulins and ftsZ in different Prosthecobacter species
external page Molecular Biology and Evolution